Urea cycle of Fasciola gigantica: purification and characterization of arginase.

Purification and Characterization of Nitric Oxide Synthase in Fasciola gigantica

Nitric oxide (NO) and NO-related pathways have the potential to play critical roles in the helminthes life cycle. Nitric oxide and nitric oxide synthase(NOS) in the parasitic trematode Fasciola gigantica were investigated. Two forms of F. gigantica nitric oxide synthase were detected after chromatography on CM-cellulose. The NOS II had the highest specific activity. NOS II was purified to homog...

Further Observation on the Susceptibility of Different Species of Lymnaea Snails of Iran to Miracidia of Fasciola hepatica and Fasciola gigantica

Determination of somatic and excretory-secretory antigens of Fasciola hepatica and Fasciola gigantica using SDS-PAGE

Human fascioliasis due to unknown species and animal fascioliasis caused by both or one of Fasciola spp. are commonly seen in Iran. To compare electrophoretic patterns of somatic and excretory-secretory antigens of F. hepatica and F. gigantica  by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the adult flukes were collected from infected slaughtered bovine livers. E/S a...

Effects of climatic factors on prevalence of developmental stages of Fasciola gigantica infection in Lymnaea snails (Lymnaea auricularia var rufescens) in Bangladesh

The present study was conducted to investigate the prevalence of developmental stages of Fasciola gigantica infection in Lymnaea snails and their populations in Sylhet region of Bangladesh. A total of 1865 Lymnaea snails were collected and examined, of which 56 (3%) were found to having infected with developmental stages of Fasciola gigantica. Only 4.08% infected of 515 snails were collected fr...

Fasciola gigantica-specific antigens: purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis.

Immunodominant antigens of an approximate molecular mass of 27 kD were obtained from an excretory-secretory product of adult Fasciola gigantica by a continuous-elution method. An indirect ELISA using the antigens obtained by this relatively simple procedure was developed for detecting specific antibodies from patients infected with F. gigantica. Sera from patients with other parasitic infection...